Strategies for HPLC Method Development - Webinar Recording

This video is a recording of a webinar presented by Oona McPolin of Mourne Training Services Ltd on the 4th August 2020. The webinar explored the different strategies, and combinations of strategies, which may be applied during HPLC method development. The best approach may vary depending on the particular requirements for a given separation. Mourne Training Services Ltd provide a range of courses on the topic of HPLC, visit the website for more information: https://mournetrainingservices.com/course_list Navigation: 0:00 Introduction 0:15 Webinar info 3:42 Who's attending this webinar? 5:56 Challenges in HPLC Method Development 7:37 One size fits all? 8:02 Choice of strategy depends on 9:27 Is your desired method... 15:21 What is your greatest resource challenge? 16:43 2 Phases of method development 17:54 Examples of strategies 19:47 Quality by Design (QbD) 20:42 Analytical Quality by Design (AQbD) 21:50 Find a method in the literature 22:55 Pros and cons 27:09 Trial and error 29:23 Generic approach 31:39 Screening experiments 32:20 Example of screening experiment 36:51 Design of Experiments (DoE) 40:34 When to use it 41:43 Changing one factor at a time (OFAT) 43:04 Example strategy for experiments 44:40 Computer simulation and modelling 45:13 Typical modelling options 47:45 Suggested 5-Step Strategy 49:53 Summary of key points