Determining the Chemical Profile of Cannabis via HPLC and HRMS Methods

Determining the Chemical Profile of Cannabis via HPLC and HRMS Methods Reference Article Berman, P., Futoran, K., Lewitus, G. M., Mukha, D., Benami, M., Shlomi, T., & Meiri, D. (2018). A new ESI-LC/MS approach for comprehensive metabolic profiling of phytocannabinoids in Cannabis. Scientific reports, 8(1), 1-15. https://www.nature.com/articles/s41598-018-32651-4 Cannabis Variability Cannabis strains significantly vary in their chemical compositions. The concentration of Cannabis’s compounds depends on the plant’s tissue-type, age, variety, growth conditions (nutrition, humidity and light levels), harvest time, and storage conditions. Analyzing the chemical content of the plants is of major importance considering that the concentrations of these constituents and their interplay may determine medicinal effects and adverse side effects. Gas Chromatography (GC) vs High Performance Liquid Chromatography (HPLC) High performance liquid chromatography (HPLC) resolves the threat of phytocannabinoid decomposition as a result of heating compared to GC HPLC can be directly applied for fractionation of extracts into separate pure compounds, unlike GC Fractionation of extracts are of high importance in medical research and for structure clarification. Reference Article Citti, C., Linciano, P., Panseri, S., Vezzalini, F., Forni, F., Vandelli, M. A., & Cannazza, G. (2019). Cannabinoid profiling of hemp seed oil by liquid chromatography coupled to high-resolution mass spectrometry. Frontiers in plant science, 10, 120. https://www.frontiersin.org/articles/10.3389/fpls.2019.00120/full Extracted Ion Chromatograms Liquid chromatography methods coupled to high-resolution mass spectrometry detection (HPLC-HRMS) have been developed for the identification of the different cannabinoids in cannabis medicinal extracts The HRMS technique can define the cannabinoid profile of cannabis end products Current focus is on the main cannabinoids, CBD and THC, along with other “minor” cannabinoids, which contribute to the final beneficial effects. Macro to Microscope Views The leaf of a typical cannabis plant Magnified leaf surface showing trichomes Surface swab of leaf Mass Spec of sample collected Link to Lecture Slides: https://drive.google.com/file/d/1MjZ1fUBtmmXWMRH0lln607z_rhV2bD71/view?usp=sharing *Due to the description character limit the full work cited for "Determining the Chemical Profile of Cannabis via HPLC and HRMS Methods" can be viewed at... https://drive.google.com/file/d/1_UQnG7ArMEPvSnPvGbeYvkt-A4KZlH-_/view?usp=sharing